Journal: Drug Design, Development and Therapy

Article Title: Cordycepin Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis in Mice by Inhibiting IL-6/IL-6R-Mediated p38 MAPK and NF-κB Activation Through Adenosine A 2A Receptor Stimulation

doi: 10.2147/DDDT.S575035

Figure Lengend Snippet: Cordycepin mitigates 2% DSS-induced damage in NCM460 cells. ( A ) Effects of different concentrations of DSS on the viability of NCM460 cells. ( B ) Effects of different concentrations of cordycepin (COR) on the viability of NCM460 cells. NCM460 cells were treated with 2% DSS and / or 1 μmol/L (COR-1), 10 μmol/L (COR-10) COR for 24 h, the release levels of ( C ) LDH, ( D ) IL-1β, ( E ) IL-6, and ( F ) TNF-α in the supernatant were measured by using Elisa. ( G ) Representative images of PI staining and the percentage of PI-positive cells, scar bar = 100 μm. ( H ) The protein expression of ZO-1 in NCM460 cells was determined by using Western blotting assay. The results were representative of three independent experiments and expressed as mean ± SEM. Data were compared using two-tailed Student’s t tests. # P < 0.05, ## P < 0.01, ### P < 0.001 and #### P < 0.0001 vs. control group; *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001 vs. the 2% DSS group.

Article Snippet: Serum CRP levels were measured using a mouse-specific sandwich ELISA kit (E-EL-M0053, Elabscience).

Techniques: Enzyme-linked Immunosorbent Assay, Staining, Expressing, Western Blot, Two Tailed Test, Control

Journal: Drug Design, Development and Therapy

Article Title: Cordycepin Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis in Mice by Inhibiting IL-6/IL-6R-Mediated p38 MAPK and NF-κB Activation Through Adenosine A 2A Receptor Stimulation

doi: 10.2147/DDDT.S575035

Figure Lengend Snippet: Cordycepin abates DSS-induced damage in NCM460 cells by activating adenosine receptor A 2A . ( A ) The cAMP levels in NCM460 cells under treatment with 2% DSS and/or 1 μmol/L and 10 μmol/L COR. ( B ) The cAMP levels in NCM460 cells under treatment with 2% DSS, 10 μmol/L COR, 5 μmol/L SCH58261 (SCH), and / or 5 μmol/L DPCPX for 24 h. The release levels of ( C ) LDH, ( D ) IL-1β, ( E ) IL-6, and ( F ) TNF-α were measured by using Elisa. ( G ) Representative images of PI staining and the percentage of PI-positive cells, scar bar = 100 μm. ( H – J ) The protein expression of ZO-1, A 2A AR and A 1 AR in NCM460 cells was determined by using Western blotting assay. The results were representative of three independent experiments and expressed as mean ± SEM. Data were compared using two-tailed Student’s t tests. vs. control group; # P < 0.05, vs. control group; *P < 0.05, **P < 0.01 and ***P < 0.001 vs. the 2% DSS group.

Article Snippet: Serum CRP levels were measured using a mouse-specific sandwich ELISA kit (E-EL-M0053, Elabscience).

Techniques: Enzyme-linked Immunosorbent Assay, Staining, Expressing, Western Blot, Two Tailed Test, Control

Journal: Drug Design, Development and Therapy

Article Title: Cordycepin Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis in Mice by Inhibiting IL-6/IL-6R-Mediated p38 MAPK and NF-κB Activation Through Adenosine A 2A Receptor Stimulation

doi: 10.2147/DDDT.S575035

Figure Lengend Snippet: SCH58261 blocks cordycepin’s amelioration of intestinal inflammation and gut barrier function in DSS-induced colitis mice. ( A – C ) Level of colonic IL-1β, IL-6, and TNF-α among the Control, DSS, DSS+COR-L and DSS+COR-H group were detected by using Elisa. ( D ) Representative images of AB-PAS staining of colonic tissue. The arrow indicates the goblet cells. ( E ) Number of goblet cells in colonic tissue. ( F ) Representative images of TUNEL staining in colonic tissue, scale bar = 50 μm. ( G ) Percentage of TUNEL-positive cells (%). ( H ) The protein expression of ZO-1 and Occludin in colons was determined by using Western blotting assay. ( I ) and ( J ) Serum level of D-lactate and diamine oxidase (DAO) were measured by using Elisa. ( K ) The serum cAMP levels among the control, DSS, DSS+COR-L, DSS+COR-H group and DSS+SCH+COR-H group. Data were presented as the means ± SEM of six-eight mice in each group and were compared using two-tailed Student’s t tests. # P < 0.05, ## P < 0.01, ### P < 0.001 and #### P < 0.0001 vs. control group; *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001 vs. the DSS model (DSS) group.

Article Snippet: Serum CRP levels were measured using a mouse-specific sandwich ELISA kit (E-EL-M0053, Elabscience).

Techniques: Control, Enzyme-linked Immunosorbent Assay, Staining, TUNEL Assay, Expressing, Western Blot, Two Tailed Test

Journal: Cell and Tissue Research

Article Title: Early intestinal barrier changes in A53T transgenic Parkinson’s disease mice

doi: 10.1007/s00441-026-04062-9

Figure Lengend Snippet: Assessment of systemic and local inflammatory markers. a No significant difference in CRP plasma levels was found when comparing between genotypes at either 12 or 36 weeks; however, both WT and A53T mice demonstrated significant age-related reductions. b No significant differences in plasma TNF-α levels were detected. c No significant differences in plasma IL-6 levels were detected; however, an age-related increase was seen in A53T mice. d Plasma LBP levels remained comparable between WT and A53T mice at both 12 and 36 weeks. e , f GFAP-immunoreactivity in the myenteric plexus of the ileum showed no genotype-dependent differences in the distal ileum ( e ) or the distal colon ( f ). g , h CD45-immunoreactivity remained comparable between genotypes in both the ileum ( g ) and colon ( h ). i Representative images of GFAP (cyan) and CD-45 (magenta) in the ileum of 36-week-old A53T mice. Statistical analysis was performed using two-way ANOVA followed by Fisher’s LSD test for genotype comparisons. Results are presented as mean ± SEM, with n = 5–8 per group

Article Snippet: Plasma samples were diluted 1:10 in plasma diluent, and TNF and C-reactive protein (CRP) levels were quantified using mouse TNF (ab208348, Abcam) and CRP (DY1829, R&D systems) ELISA kits following the manufacturer’s protocol.

Techniques: Clinical Proteomics

Journal: Frontiers in Immunology

Article Title: Melatonin alleviates airway inflammation and anxiety-depression in asthma via gut microbiota–SCFA axis-mediated inhibition of microglial activation

doi: 10.3389/fimmu.2026.1763305

Figure Lengend Snippet: Melatonin/sodium butyrate/fecal microbiota transplantation alleviate anxiety and depression in asthmatic mice via the MAPK/P65/NLRP3 pathway. (A, B) Unbiased pathway analysis revealed significant differences in the activation of the MAPK signaling pathway and the NLRP3 inflammasome pathway. (C, D) Western blot analysis of key proteins in the MAPK/P65/NLRP3 signaling pathway in hippocampal tissues. (E, F) Quantitative analysis was performed on the protein expression levels of the MAPK/P65/NLRP3 signaling pathway in hippocampal tissue extracts from mice. Data are presented as mean ± SEM. ns, not significant ( p > 0.05), * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Mouse Reactive Inflammasome Antibody Sampler Kit , CST , 20836T , WB: 1:2000 IF:1:200.

Techniques: Transplantation Assay, Activation Assay, Western Blot, Expressing